We offer DNA sequencing of single stranded or double
stranded DNA samples from purified plasmids and PCR products.
Our sequencing reactions are performed using the Applied Biosystems Big Dye Terminator V3.0 sequencing chemistry. One sequencing
reaction consists of one DNA template (customer supplied
plasmid DNA or PCR product) being sequenced with one primer
(customer supplied primer or one of our supplied primers).
We supply several frequently used sequencing
primers for free, but the template DNA samples are
supplied by our customers.
Our sequencing reaction read-lengths are normally between
800 and 900 bases (with a basecalling accuracy >99%) when run on our ABI 3730 DNA sequencers. Sequencing read-lengths are
dependent upon several factors including template DNA
concentration, purity and base composition (poly A and/or
T regions, GC content and short tandem repeats) as well
as primer binding specificity.
Since DNA sample purity and concentration are the two
most critical factors to
the success of automated sequencing reactions, we highly
recommend that customers use the QIAGEN® plasmid DNA prep kits to purify their
plasmid DNA samples. For purifying PCR products, we recommend
using either the QIAquick Gel Extraction Kit (supplied by QIAGEN®) or Centricon®
100 columns (supplied by Amicon®) to remove unincorporated
dNTPs and primers. In our experience, other DNA purification
kits and methods are less reliable for preparing automated
sequencing quality DNA samples. Please call us if you have any questions regarding the compatibility of other DNA sample preparation methods with our DNA sequencers.
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